BI309: Orchid’s Propagation Of Phaius Tancarvillaea Species Using Innovative Tissue Culture Technique

NORAZIHANIM BINTI RAHIM KOLEJ VOKASIONAL (PERTANIAN) TELUK INTAN

MIIX24 | Beginner Innovator

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Phaius tancarvilleae is a species of orchid that rarely found in Peninsular Malaysia. This species is threatened with extinction due to habitat destruction caused by natural disasters and pollution. As we know, orchid have a slow life cycle and its seed also hard to find. To solve this problem, an initiative to propagate this orchid has been carried out by using innovative tissue culture technique. In this project, Phaius Tancarvilleae is cultured from the explant stage to shoot multiplication using media contains macronutrients, micronutrients, vitamins and Plant Growth Regulator (PGR). To increase growth of Phaius tancarvilleae culture, Plant Growth Regulator (PGR) which is cytokinin, 6-Benzylaminopurine (BAP) is used. The selected part of the explant is seed. Data analysis method used to obtain the results is randomized complete block design (RCBD). Based on the result obtained, the weight of callus is 1.682 gram for replication 1 and 1.564 gram for replication 2 is the best result compared to the other treatment. As for shoot multiplication, the number of shoot is 7 shoot for replication 1 and 8 shoot for replication 2 is the best result compared to the other treatment. This innovative tissue culture technique can produce a lot of plantlet in a short time compared to conventional technique. In addition, this technique also can reduce extinction for Phaius tancarvilleae orchid.