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Conventional treatment of palm oil mill secondary effluent (POMSE) unable to remove the colour and organic load that not meet the requirement of discharge standard limit to the water course. The main reason using green synthesis of ZnO is because it was eco-friendly so can avoiding the utilization of harmful synthetic compounds, easy to find it especially in Asia, and a high cost-effective method. In this present paper, ZnO NFs were synthesized through a green route by means of using Pandanus Amaryllifolius Roxb. leaf extract (PAR) and then dispersed in a mixture of choline chloride to glycerol with ratio (1:2, 1:4 and 1:6). Usually, PAR leaf extract was a capping agent that are widely employed to functionalize and stabilize the synthesized ZnO to ZnO NFs. Nanofluids are easier to handle and mix in wastewater systems compared to dry nanoparticles, which can be difficult to distribute evenly without specialized equipment. Their liquid form allows them to be pumped and injected directly into treatment systems. Therefore, the main objectives of this work were to formulate zinc oxide using ZnO-PAR NFs under different molar ratio of deep eutectic solvent (DES) based nanofluid and to elucidate the ideal performance of various ZnO-PAR NF formulations in treating POMSE via MPR.