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This research seeks to develop an innovative 3D-printed biosensor for the rapid and accurate detection of E. coli bacteria. Existing techniques are often labor-intensive, expensive, and time-consuming. The proposed biosensor will incorporate a flow cell design, which allows continuous monitoring and efficient detection in real-time. It will be fabricated using conductive polymer-based materials to ensure high sensitivity and specificity. The research will proceed through several key phases: designing and simulating the flow cell structure, selecting and functionalizing the sensing materials, fabricating the biosensor using 3D printing techniques, and conducting extensive testing with water samples containing various concentrations of E. coli.