MIIX Virtua

Experimental model of glaucoma often associated with visual deficit. Notably, both optic nerve grading and retinal layer assessment are two common methods in assessing the eye structure. However, these two methods alone are not enough estimate the visual deficit. Hence, the two-mirror chamber test is a tool to help in estimating the time spent for the visually impaired rats in the chamber allocated. Rats were placed into the central bridge and then given 5 min to freely explore the apparatus. Rats were then removed from the apparatus, and after 5 min of rest, they were subjected to a retest for another 5 min with the apparatus rotated 180 degrees to swap the positions of the two chambers to exclude the effect of learning and memory. In conclusion, detection of visual impairment is observed through rat’s indifference on both chambers through this tool